I have two question about MTT assay
1. The medium for MTT assay should been added FBS?
Someone told to me that FBS can help cell proliferation. Will it effect the accuracy?
2. When i read papers about MTT assay,i found many experiments that treat cells in the same dose of drug for different times.
What are the meaning of different treatment times? (eg. 3, 6, 12, 24 hours)
Do the different times mean acute or chronic toxicity?