Help with positive control
Posted 24 October 2013 - 06:25 AM
So I still needs help, I need another positive control, this control can be a gene that it's full methylated, where I can find a list of possible candidates.
And what it's more useful, this or using a ssi methyltransferase kit to make my own methylated controls
Posted 24 October 2013 - 07:48 AM
Another possible gen?
Posted 29 October 2013 - 09:56 AM
Question -- can you describe why you intend to use a specific gene as the positive control for a global DNA methylation study?
Posted 29 October 2013 - 01:22 PM
You can make a fully methylated sample with PCR using 5me-dCTP instead of CTP. You can also methylate only the CGs of a DNA sample with M.SssI (NEB).
Posted 18 November 2013 - 01:52 AM
Indeed, full methylation of a gene often depends on the cell line or tissue you are looking in. Not a reliable 'control' . Go for enzymatic methylation for the 100% methylated and a whole genome amplified DNA for a 0%, you can than make a titration curve for your global meth analysis, much more insightful maybe....