Hi to everyone!
I really need your help. I´m starting in a new lab and I making some WB, which I made thousands of them during my predoc term but I´m getting some problems, This is the fifth time I made this WB and I´m getting crazy right now because I made new solution for everything.
Let me give you some inf: White adipose tissue samples, lysis buffer (Hepes 50mM, NaCl 200mM, Glycerol 5%, Triton X100 1%, Ortovanadate, Naf, Complete), 9% acrilamide, 25uL sample into 1mm BioRad Gels, around 20ug protein, runing at 100V.
Please, see the pic and you will see a "white ghost band" in the middle of the gel, and I do not what is this because I am using the same protocol since 4 years ago, I just change the lab... (maybe some negative energy... hehehe, joke)
Please, please, and please, could you help me to resolve this problem? Have you ever seen something like this?
Thanks so much in advance!!!!
Edited by Vemovied, 24 October 2013 - 05:24 AM.