We ordered NsiI FastDigest from Fermentas. It is supposed to share the buffer with NdeI which is from Biolabs. I tried using FastDigest Buffer which is the buffer that came in with NsiI and NEBuffer 3.1 which is the buffer for NdeI, none of them works at optimal condition. Only a small fraction of the template was cut by both of the enzyme...
I did single digests to check their activity and both of them worked fine in their separate buffer.
Anything else I could do other than ordering new RE?
Edited by Celeste, 23 October 2013 - 01:30 PM.