Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
* * * * * 1 votes

expressing flag tag by itself

flag tag expression promoter kozak translation

  • Please log in to reply
1 reply to this topic

#1 kaveh

kaveh

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 37 posts
2
Neutral

Posted 22 October 2013 - 02:08 PM

I was wondering if it's possible to express flag tag all by itself. I was thinking about putting the flag tag (with a kozak sequence in the beginning and a transcription terminator in the end) downstream of a promoter and upstream of a poly-A signal. Would this lead to expression of a short peptide that could be recognized by antibody?

 

thanks.



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,786 posts
406
Excellent

Posted 22 October 2013 - 08:03 PM

Short answer is yes, however, the peptide will be tiny (6 kDa I think) and as such quite hard to detect by conventional western blotting techniques.  You may be better off using a 3x flag tag.







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.