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expressing flag tag by itself

flag tag expression promoter kozak translation

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#1 kaveh



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Posted 22 October 2013 - 02:08 PM

I was wondering if it's possible to express flag tag all by itself. I was thinking about putting the flag tag (with a kozak sequence in the beginning and a transcription terminator in the end) downstream of a promoter and upstream of a poly-A signal. Would this lead to expression of a short peptide that could be recognized by antibody?



#2 bob1


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Posted 22 October 2013 - 08:03 PM

Short answer is yes, however, the peptide will be tiny (6 kDa I think) and as such quite hard to detect by conventional western blotting techniques.  You may be better off using a 3x flag tag.

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