Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Removing ~200 different transcripts from total RNA pool

  • Please log in to reply
1 reply to this topic

#1 Ahrenhase



  • Awaiting Authorisation
  • PipPipPipPipPipPipPipPipPipPip
  • 196 posts

Posted 22 October 2013 - 10:18 AM

I was going to mix antisense oligos, directed against the start codon region of the mRNAs I want to remove, and RNase H into purified total ply(A) mRNA.  I'm thinking this approach is a bit sloppy, though, and I'd have to validate ~200 oligos. I was wondering if there was an easier way:


My transcriptome of interest contains roughly 15000 different transcripts, so piecing together a new transcriptome, without the 200 transcripts I want to remove, isn't feasible.

Edited by Ahrenhase, 22 October 2013 - 10:19 AM.

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,670 posts

Posted 22 October 2013 - 12:26 PM

How about binding oligos to beads and then pulling down?

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.