Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Barcodes with Illumina MiSeq


  • Please log in to reply
1 reply to this topic

#1 LabNerd

LabNerd

    member

  • Active Members
  • Pip
  • 8 posts
0
Neutral

Posted 22 October 2013 - 06:41 AM

Dear all

 

We are an environmental microbi group who's interested in pooling samples for 16S metagenomic work on the Illumina Miseq. We understand that the Illumina kit contains 96 unique barcodes, hence that is the maximum number of samples we can combine into a reaction. However, we would like to pool more samples (mainly because we have a LARGE number of samples and would like to minimize cost by doing less runs).

 

We know service providers that can do up to 144 samples, but does anyone here know providers that can do more than that? We know that it's a balance between number of sample per run and reads per sample, but we're curious as to whether there are providers out there who have enough unique barcodes for more than 144 samples per run...

 

Thanks so much!



#2 firefly2280

firefly2280

    member

  • Active Members
  • Pip
  • 15 posts
0
Neutral

Posted 28 February 2014 - 01:27 AM

Hi, check out this link, there are over 2000 barcodes you can use! 

 

http://www.earthmicr...-protocols/16s/






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.