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Step-by-step protocol for newbie in gene expression analysis

affymetrix gene expression microarray analysis

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#1 LabDiagMol

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Posted 22 October 2013 - 02:13 AM

Hi everyone,

 

I need your help to get some detailed information regarding microarray data analysis for gene expression profiling (affymetrix genechip Human Gene 2.1 ST arrays). I'm absolutely newbie in this field and I've basic skills in R/Bioconductor.

 

I've been taking a look into Bioconductor website and I already have the packages used in microarray analysis workflow, but I feel that's not enough for me. I need a step-by-step tutorial/protocol.

 

Do you know where can I find such kind of information?

 

 

Thank you in advance!

 

 

 



#2 mdfenko

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Posted 22 October 2013 - 03:29 AM

this manual (pdf from the affymetrix website) will give you the protocols you require:

 

geneatlas wt expression kit user manual


talent does what it can
genius does what it must
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#3 LabDiagMol

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Posted 22 October 2013 - 04:10 AM

thank you mdfenkofor your advice, but what I really need is some kind of software protocol for data analysis in R/Bioconductor

 

Best,



#4 mdfenko

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Posted 23 October 2013 - 04:18 AM

here is an on-line manual

 

and

 

the bioconductor help page

 

these should give you what you need


talent does what it can
genius does what it must
i do what i get paid to do





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