Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

unidentified objects in my cells, no staining.. what are they?

microscopy cell immunofluorescence contamination fluorescence

  • Please log in to reply
2 replies to this topic

#1 bidinozor

bidinozor

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 19 October 2013 - 02:15 PM

I did not stain my cells, I just looked them under fluorescence microscope with blue filter usage, so each time I see a bright nucleus and somethings distrubuted in cytoplasm...(see photograph on link or attachment)...

 

I want to use blue dye with other dyes for staining but these cell are not appropriate for this purpose because of bright nucleus especially 

 

any idea? I am lost

 

https://fbcdn-sphoto...af9a4d40a80d8f6

Attached Thumbnails

  • 1394859_10151952789952220_1564603189_n.jpg


#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,674 posts
396
Excellent

Posted 19 October 2013 - 03:16 PM

Those things are probably subcellular components like mitochondria or endoplasmic reticuluum if you didn't stain with DAPI. When you stain your cells these should not be apparent with the exposure times you should use. If you continue to see faint spots in the cytoplasm, these are likely to be mycoplasma contamination, and as such indicate that your cells are not healthy and should be discarded.

BTW using a blue light filter (i.e. so that you see a blue light shining on your slide) will give you fluorescence in the green range of colour, not blue.

#3 CellApplicationsInc

CellApplicationsInc

    Sr. Product Scientist

  • Active Members
  • Pip
  • 21 posts
2
Neutral

Posted 25 October 2013 - 01:21 PM

look like mitochondria to me. Try staining them with DAPI, and, like Thelymitra suggested, your exposure time will likely be so short, that the autofluorescent structures will not be a problem.







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.