I don't know much about immunology, just remember very few basic things, that I had read long back . For some of my work, I was reading about generation of antibodies. Adjuvants play a major role in this. And in mice/rabbit I read that mostly FCA is used. From my basic understanding, I know that adjuvant helps immune system in recognition and elicit immune response. Now I have a question, what if the antigen itself is strong enough to be recognized by immune system, do we still need adjuvant?
Also I was reading about various types of adjuvants, and came across to CpG. It is not emulsion based and just a dinucelotide. If antigen is very labile and mixing with adjuvant is a problem (as it needs vigorous mixing), can this CpG be used?
Please correct me if I am wrong somewhere and clear my doubts.