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18 s thicker than 28 s


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#1 student47

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Posted 17 October 2013 - 03:15 AM

hi all,

what is the logic behind that after isolation of rna the 28 s needs to be thicker than 18 band in rna iaolation??



#2 phage434

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Posted 17 October 2013 - 07:27 AM

I think you mean by "thicker" that the band has more RNA, and is brighter.

Ribosomes are composed of one small and one large subunit, so the number is roughly equal in every cell. Since the intensity of the band is proportional to the mass of the RNA, and the 28S is longer, it should have higher mass, and be brighter.  If not, this likely indicates that some of the 28S RNA has been cleaved by RNAse contamination.



#3 student47

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Posted 17 October 2013 - 07:57 AM

i thought so but needed to hear it from someone. thanks..






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