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Nuclei fixation using formaldehyde

formaldehye 4C ChIP fixation

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#1 Kriti Khandelwal

Kriti Khandelwal

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Posted 16 October 2013 - 12:44 PM

Hi guys,

 

Normally I guess we use 1 % or 2 % final conc.of formaldehye for fixing the interactions between dna-dna and dna-protein in our 4C and ChIP experiments. I have instead of 1% used 10% final conc of formaldehye.

 

What should I expect in following steps?
 

Of course, I can expect the complete loss of DNA in phenol choloform after proteinase K treatment. Because if normal prot k treatment is not enough than all thye DNA will be lost in the phenol cholorofom step.

 

What should I do now?

 

NMM







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