Normally I guess we use 1 % or 2 % final conc.of formaldehye for fixing the interactions between dna-dna and dna-protein in our 4C and ChIP experiments. I have instead of 1% used 10% final conc of formaldehye.
What should I expect in following steps?
Of course, I can expect the complete loss of DNA in phenol choloform after proteinase K treatment. Because if normal prot k treatment is not enough than all thye DNA will be lost in the phenol cholorofom step.
What should I do now?