Recently I attempted a tumor innoculation using MDA-MB-468 cells combined with Matrigel , the suspension was a 1:1 volume to volume mixture, and the final suspension had 2X10^6 cells in the total 200 uL volume injected.
I injected the dual flanks subcutaneously , and once I penetrated into the SC I made a small pocket with the syringe, then while slowly injecting the cells I moved the needle to the left and right in a fan motion to spread the cells.
Now, it has been 2 weeks since the innoculation, and you can see a very small lumps that are very spread out.
Is it better to inject it directly as one mass without moving the syringe needle? Also, should I have put much more cells because the growth is really small and limited?
What do you guys recommend, can somebody give me some tips? Was it such a small volume that was dispersed?
I was thinking to inject again using 200uL of the 1:1 mixture with matrigel with a total of 5X10^6 cells directly as once lump?
When you guys penetrate the SC and form a pocket do you inject all the cells together as a lump, or do you sway the needle left and right to deposit the cells over the entire suface to increase the surface area?
I have had really bad luck with this tumor induction and would really appreciate some tips from you guys!