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Growing E.coli under anaerobic conditions with ethanolamine

anaerobic e.coli ethanolamine

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#1 Hazraa

Hazraa

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Posted 15 October 2013 - 06:04 PM

Hi!

 

Has anyone grown E.coli K-12 under anaerobic conditions using ethanolamine as the sole nitrogen source or even carbon and nitrogen source with cobalamin?

 

E.coli has the enzyme ethanolamine ammonia lyase which requires cobalamin (B12) to break it down to produce ammonia and acetate. I have  successfully grown E.coli K-12 in ethanolamine (N source) + glycerol (C source) with added B12 under aerobic conditions. I have also grown E.coli K-12 in ethanolamine (C and N source) with added B12 under aerobic conditions. But when I try to grow E.coli K-12 anaerobically with ethanolamine + glycerol, it does not grow.

 

I realize this has something to do with the electron donor and acceptor that I have to provide E.coli with under anaerobic condition. I would appreciate any inputs from the community on what electron donor-acceptor pair works for ethanolamine growth of E.coli under anaerobic conditions.

 

Thanks much!







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