I am about to start work on a project.
I will be using cAMP and gAMP analogues to stimulate cholesterol efflux in macrophages.
I have to prepare my drugs to a concentration of 0.1mM in DMSO.
The stock of each drug can have no more than 5ul per 1000ul of DMSO.
The MWt of the cAMP is 430.09 and the cGMP is 500.14.
The testing will use 20ul of stock drug into 1ml of media containing macrophages.
The cGMP I has come on a bottle in powder form (10mg), looking at it I will probably get about 2mg out to use.
So if I wanted to make a stock solution to say 1mM I would weight out 500.14mg and add it to 1ml of DMSO?
So when using my stock at 0.1mM I would make a 1:10 dilution from 1mM stock?
Sorry if this is basic I’m just getting confused and probably over complicating things.