Hi, I have experience with cell culture but having trouble starting a culture od SH-SY5Y cells and need advice.
I have tried putting in culture a vial of cells with 3 types of media, all suitable (DMEM, DMEM/F12 or EMEM/F12) containing 10% FBS, NEAA, glutamine and antibiotics. Media were high glucose, with sodium pyruvate and sodium bicarbonate. I have tawed the criovial and added the cells to a 75cm flask, had lots of cells looking under the microscope, replaced the media after 24h, and noticed I had less and less cells in the flask every day. Am I making any very basic mistake with these cells?