Hi all, I have been attempting some soft agar assays but have had contamination the second day after plating. After 3 attempts (and nothing else getting contaminated- other assays, cell cultures, etc) I am convinced it is not my technique but that the agarose is contaminated and needs to be sterilized. I am using low melt temp agarose (only opening it under hood) and dissolving it in sterile DMEM in a 65 deg water bath.
So my question is, how can I sterilize the agarose? Can I filter it, and if so what pore size (or will the agarose just clog up the filter?) Or can I autoclave it (or is this too hot and will it break down the agarose?). Any help would be greatly appreciated, thanks!