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NFAT nucleocytoplasmic shuttling


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#1 paulis



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Posted 13 October 2013 - 01:53 PM

hello everybody
I write for help, and I need to locate the NFAT protein in the nucleus of neuroblasts through immunofluorescence, for which I need a stimulation of these. I have long been trying without remarkable results. I consulted papers, they suggest lithium (in my lab are: Li2CO3, and NGF (nerve growth factor), but none of them is so clear in the process, I would like someone to help me please with the concentrations and especially with the incubation time necessary, thank you very much
in advance

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