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DNA extraction , phenol , ice . plz help !


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#1 M_shabani_medbiotech

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Posted 13 October 2013 - 06:04 AM

hi every one , i wanna know why we put our solution on ice after we add phenol to it . blink.png 

 

any help is appreciated 

 

XOXO smile.png 



#2 bob1

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Posted 13 October 2013 - 11:42 AM

As far as I know there isn't any need to ice during the phenol step (unless perhaps you are working on something with a lot of DNases).  I have done many a DNA phenol extraction where the only time I would use ice is during the DNA precipitation.



#3 Shefali Desai

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Posted 13 October 2013 - 08:23 PM

You do not need to keep on ice you get better separation at RT. Even for  precipitation RT works better. 



#4 M_shabani_medbiotech

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Posted 14 October 2013 - 06:47 AM

my supervisor told me so blink.png

Thanks guys wub.png






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