you know when you use a enzyme to cut your self ligated vector background by choosing a site in your vector that is not there in your insert. hope you know what am talking about else tell me and i will elaborate.
when i add the cut enzyme, most people say just add a microlitre at end of ligation and incubate at optimum for around 10 minutes, and it should work, is that true? and also
well when you do that, do you need to heat inactivate the enzyme at the end of it? , if i do it might also affect the transformation? can i just leave it and transform, will it inhibit tranformation?