Apologies for posting a basic questions.....
I have 25mg powder in my tube, mol weight = 258.23. It needs to be reconstituted in water at min 7mg/ml.
I want to have a six well plate with 0.5mM, 1mM, 2mM and 5mM solutions in the wells.
By my calculation - need to dissolve 3.6ml water
Total moles in tube = 25*10-3 / 258.23 = 0.00096 moles
For 0.5mM well, need 0.5*10-3 * 2*10-3 = 1*10-6 moles (assuming that I put in 2 ml of media in the well)
Therefore I need 1*10-6/0.00096 = 0.001* 3.6 = 3.5 microlitre of the original solution in 2 ml of media in the well to make 0.5mM
is this right?