Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Wrong PCR product

Hello every one

  • Please log in to reply
2 replies to this topic

#1 vpvatuan

vpvatuan

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 11 October 2013 - 05:31 AM

I have some problem with my PCR product. I design primer base on invitrogen. The expected size of my PCR product is 290bp. I do with RNA (RT-PCR one step). But The band is very nice but the size about 400bp. Please give me some advice? Thank you

 



#2 Tabaluga

Tabaluga

    And we're back to square one

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 315 posts
43
Excellent

Posted 13 October 2013 - 08:20 AM

Your primer might recognise another gene than the one you want to detect, giving a completely different fragment. Try with a different pair of primers. An alternative might be designing primers with PrimerBLAST or looking for ones that have already been used in publications.


Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 


#3 vpvatuan

vpvatuan

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 20 October 2013 - 07:55 PM

Thank you Tabaluga, I try to do primerblast but it not similar with another gene. 






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.