The majority of fluorescence microscopes, especially those used in the life sciences, are of the epifluorescence design shown in the diagram. Light of the excitation wavelength is focused on the specimen through the objective lens. The fluorescence emitted by the specimen is focused to the detector by the same objective that is used for the excitation which for greatest sensitivity will have a very high numerical aperture. Since most of the excitation light is transmitted through the specimen, only reflected excitatory light reaches the objective together with the emitted light and the epifluorescence method therefore gives a high signal to noise ratio. An additional barrier filter between the objective and the detector can filter out the remaining excitation light from fluorescent light
Ok, what does this red/bold part mean?
the excitation light, this is the light created by the laser (the light source, right?)
And the reflected excitatory light, what is this? This is the light created by the excitation of the fluorophores?
But what is the emitted light than? I was thinking this is also the light created by excitation of the fluorophores, but that does not seem to be the case?
(could be the wrong place to ask this, if so: maybe a moderator can move it.)