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How Do You Determine What The Electron Acceptor/Donor In Your Media Is?

Media Anaerobic

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4 replies to this topic

#1 SunsetSatine

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Posted 09 October 2013 - 06:23 AM

I have the following media that I used for an enrichment:

 

Tryptone

Yeast Extract

Starch

NaHCO3

Rumen Fluid

Mineral Solution

DI Water

 

The media was prepared anerobically. How do I figure out where my electron donor/acceptor came from?

 

I would really appreciate any help!



#2 El Crazy Xabi

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Posted 09 October 2013 - 05:47 PM

Without knowing more about the output, you will have fermentation and may also have methanogenesis.

 

Also, even if you prepared the media anaerobically, if you didn't add any reducing agent to remove all the oxygen there will be some oxygen remaining in there. Not very much and it may be depleted quite fast, but you may have some at the beginning



#3 SunsetSatine

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Posted 10 October 2013 - 09:57 AM

Whoops! Forgot to mention that I added Cystein-Sulfide as the reducing agent to remove any residual oxygen. What do you mean by "output?"



#4 El Crazy Xabi

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Posted 10 October 2013 - 04:35 PM

Any results or observations you made about the culture



#5 SunsetSatine

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Posted 11 October 2013 - 05:36 AM

Oh! Well, it was an enrichment that I inoculated with my sample. I let it incubate and then took a small sample of that and inoculated them onto BHI + Blood plates.







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