my amp res plates after ligation dont have any colonies after overnight in 37 deg c, or have toooo small colonies, i wish to leave them longer in 37 to find more growth. how long can i leave the plates there before effects like plasmid loss and stuff happens?
how long for bacteria in 37 deg C?bacteria plates
Posted 05 February 2014 - 01:28 PM
If you're growing at 37C, max I'd incubate for 24 hours. If your colonies are super tiny or have hardly any then your clones probably didn't work.