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how long for bacteria in 37 deg C?

bacteria plates

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4 replies to this topic

#1 student47

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Posted 09 October 2013 - 12:05 AM

my amp res plates after ligation dont have any colonies after overnight in 37 deg c,  or have  toooo small colonies, i wish to leave them longer in 37 to find more growth. how long can i leave the plates there before effects like plasmid loss and stuff happens?



#2 Missle

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Posted 09 October 2013 - 04:06 AM

If you don't have good colonies after 24hrs then your likely not going to get any.  I wouldn't trust any colonies that appeared after that....



#3 kedar

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Posted 09 October 2013 - 12:03 PM

i agree with Missle. It's true. You can trust us on this one.



#4 student47

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Posted 12 October 2013 - 03:20 AM

thanks fellas.



#5 acidfast

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Posted 05 February 2014 - 01:28 PM

If you're growing at 37C, max I'd incubate for 24 hours. If your colonies are super tiny or have hardly any then your clones probably didn't work. 






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