Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

storage for lyophilized primers


  • Please log in to reply
1 reply to this topic

#1 luckyjade

luckyjade

    member

  • Active Members
  • Pip
  • 21 posts
1
Neutral

Posted 08 October 2013 - 10:11 PM

Hello all,

 

I usually keep my lyophilized primers sitting on the bench at room temperature. Today I accidentally put them into the -20C for about 2 hours. My question is, is it okay to freeze lyophilized primers? Or I guess in my case, "freezing" for 2 hours?

 

How can I test to see if it is okay? Will a simple PCR reaction using that primer work? If PCR bands show up does that mean the primer is fine? Or could it still be that the primer is not okay?


Edited by luckyjade, 08 October 2013 - 10:17 PM.


#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,738 posts
400
Excellent

Posted 09 October 2013 - 01:18 AM

They will be fine.  It is recommended that you store lyophilized primers at fridge or freezer temperatures anyway.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.