I'm researching about function of a small chemical and I'm thinking it could interact some proteins.
This chemical is visualized in UV light, so I incubated this chemical and protein lysates and then eletrophorated in native acrylamide gel.
But I could not detect any diffrence compared to negative condition.
I think crosslink step is required but can not find any methods about protein-chemical crosslink.
1. Please advise for proteins and chemicals cross-linking methods
2. My lab has UV cross-linker. That can be used for protein-chemical crosslinking?