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Reference gene to normalize proliferation

Reference Gene Cell Cycle Normalization DNMT Proliferation Marker

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#1 Epigeneticist

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Posted 08 October 2013 - 05:34 PM

I normally use 18s rRNA or GAPDH as a reference gene to normalize expression for my gene of interest with qRT-PCR. However, I want to normalize the expression of DNMTs against a proliferation marker such as PCNA. Does anyone have experience with using such a reference gene to normalize for proliferation/cell cycle? PCNA comments or other recommendations?







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