I am a first year PhD student.
I have a calculation question:
I have to culture cells in 1 ml and then I have to add some stimulators and enhancers. I have 3 of these stimulators and the final concentration in the cell culture for each stimulator should be: Stimulator A: 1.2 mg/ml; stimulator B: 1 mg/ml; stimulator C: 2 mg/ml.
If I make the calcultation for stimulator A (stock concentration is 120 mg/ml), then I have to add 10.16 microlitres, following the equation V1 x C1 = (V2 + V1) x C2:
V1 x 120 mg/ml = (1ml + V1) x 1.2 mg/ml
But now I have to add stimulator B (stock concentration 100 mg/ml) and I have a volume of 1011.6 microlitres in the cell culture due to the volume added with stimulator A. Doing the same equation:
V1 x 100 mg/ml = (1.011 ml + V1) x 1 mg/ml
That makes 10.2 microlitres of stimulator B that I have to add.
But the problem now is that I have a increase of 10.2 microlitres in the volume of my cell culture due to stimulator B, therefore stimulator A is not at 1.2 mg/ml anymore due to the increase of volume with stimulator B. And then the same problem when I add stimulator C.
Can anybody help me with these calculations?
No need to triple post things - I've deleted your other two identical posts! Bob.