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CFSE staining of adherent cells for cell proliferation assay


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#1 sssss

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Posted 08 October 2013 - 10:41 AM

Hi,

   I have recently started with CFSE cell proliferation assay of NIH3T3  stable cells. One of my colleagues informed that high concentration of CFSE or incubation for long hours affects the proliferation and differentiation of cells (especially PBMC derived cells). Is it true for other cells (like NIH3T3 cells) ? If so please help me with the protocol for CFSE staining in NIH3T3 cells.






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