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human LL37 ELISA

elisa ll37 background

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#1 swebus

swebus

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Posted 08 October 2013 - 12:04 AM

Hi!

 

Has anyone ever done a sandwich ELISA with human LL37 peptide?

 

LL37 is a cathionic peptide and sticks to anything in the wells, which makes it impossible to get a specific signal.

 

I have tried alot of different blockers and incubation buffers like BSA,FBS, Gelatine, Water, different detergents and so on, but nothing helps.

 

Anyone got any idea?

 

 

 

 







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