Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Copy number GOI

  • Please log in to reply
No replies to this topic

#1 dceligema



  • Members
  • Pip
  • 1 posts

Posted 07 October 2013 - 11:29 AM

Hi, I'm performing a qPCR to detemine the copy number of a GOI in CHO cells. I`m doing absolute quantification and the standard curve is a serial dilution of a plasmid containing the GOI.

When I do the maths, calculating the copy number from the Ct of the sample (genomic DNA) from the regression line of the standard curve, the result is very very high. So my question is how is the correct way of calculating the copy number of GOI in eucariotic cells (CHO).





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.