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Conversion of units please help..for IC50

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4 replies to this topic

#1 Praitibha Batra

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Posted 06 October 2013 - 05:33 AM

Hi I woukd like to know how  to exactly convert 20 ug of captopril  used for inhibition activity to nanogram/litre amount..I have a vague idea ......please let me know if I am in the right direction....

 

(amount use in ug / (molecular weight in (ug/ugmole) * amount ofreaction volume in ml)= ug mole/ml

and then I can convert to nanogram/litre is it right?? please let me know...soon ..


Edited by Praitibha Batra, 06 October 2013 - 05:33 AM.


#2 phage434

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Posted 06 October 2013 - 07:49 AM

What can moles have to do with this. You have grams, you need grams/liter. Nowhere do moles come into the calculation. And what, pray tell, is the unit ug/ugmole?

 

It is very simple. You have 20 ug of captopril. You add it to some liquid (say 10 ml). The solution is now at a concentration of (20 ug)/(0.01 liters) =2000 ug/liter = 2e6 nanograms/liter.

No moles anywhere to be seen. Not even to be whacked.



#3 Praitibha Batra

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Posted 06 October 2013 - 09:19 AM

Hey sorry..ug is ( μ grams) and I  asked the question wrong if I want to convert it to  μmolar...the formula I have given is it right ?just wanted to enquire.. I understand conversions can be made..thank you.



#4 bob1

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Posted 06 October 2013 - 11:43 AM

So do you want nanomol/litre or umol/litre?

 

Not that it makes much difference... the easiest way to convert these things is to do it in two separate equations, both very simple:

 

n=m/mr (number of moles = mass/molar mass)

 

and

 

c=n/v (concentration= number of mol/volume)



#5 Praitibha Batra

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Posted 30 October 2013 - 09:22 AM

Thanks a lot everyone for the response :)







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