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Plasmid on blotting paper


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#1 science noob

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Posted 05 October 2013 - 05:04 AM

I've just received a plasmid blotted and dried on a blotting paper from an overseas lab through the air mail.  

 

1. What is the best temperature to store it in before I find a time to reconstitute it?

 

2. How long can it be stored on paper?

 

3. What is the common reconstitution buffer to obtain the plasmid DNA?



#2 GNANA

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Posted 05 October 2013 - 05:18 PM

I once happened to store them around 3 months at 4 degrees before reconstitution.

 

I normally cut the plasmid spot, put them in 1.5ml eppendorf, add around 100-200 microlitres of H2O (or T.E) and keep in shaker for few hours to overnight and store them at 4 until i transform.


I would prefer being perfectionist rather than a passionist in Research.

I always had an alternate hypothesis....




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