I am using the ATCC mycoplasma detection kit 30-1012K and am getting a band that is higher than their included positive control.
Does anyone know if that is indeed a positive or some non-specific amplificaiton? I don't get this 450bp band everywhere, just in some cells, but the positive control runs about 350bp, which is confusing the heck out of me.
Some cells, presumably the infected ones, do give the same size band as the positive control as expected.
Does anyone else get this higher 450bp band, and does anyone know if it is really mycoplasma contamination? I wanted to send it for sequencing, but can't since the primers are proprietary.
Thanks for your help