I am new to the forum, and so do I in recombinant protein expression in eukaryotic cells :-)
I am trying to set-up a recombinant protein expression in my lab, with HEK293 cells and CHO cells (expression vector: pcDNA3). For the moment I will try transient expression.
I was wondering if someone could advice to me a good positive control (I mean a protein) to validate my culture and transfection procedure. I mean, can someone tell which protein will be expressed for sure with pcDNA3/HEK293 or pcDNA3/CHO ?
Thanks a lot for your help.
PS: sorry for my poor english.