I am planning to perform a ChIP assay using an Enzymatic ChIP kit from cell signaling (#9002).
In short, the kit uses micrococcal nuclease instead of a sonicator to digest DNA.
After digestion, the protocol instructs to either use a sonicator or a dounce to break the nuclear membrane to release cross-linked chromatin.
My question is, from your experiences, would running the lysates through a 26 or 27 guage needle about 10 times be as efficient as a dounce to sheer nuclear membrane? Also, are there any precautions that I may have overlooked when using a needle to sheer nuclear membrane?
The sample of interest is a very slow growing cell line and will barely grow enough cells (recommended 4x10^7) for an experiment and trying to avoid testing different sheering methods.