I am culturing RAW 264.7 cells and recently i found cells are not growing fast and producing lot of metabolites. after i changed the culture media within 12 hrs they are producing lot of metabolites and not growing well and after 24 rs cells starting to die. cells also showing morphological changes too.
Please if anyone know the reason help me to overcome this problem. now my frozen cell store is also going to be finished.
Thank you very much