I've got trouble in analyzing DNA sequencing.
I sequenced PCR products with forward primer or reverse primer, and now I analyze these results. I compare them with NCBI genbank genome to study polymorphic gene, and find some mutations.
I want to ask you: "Can I find the frameshift mutation if I compare them with NCBI genome?", because you know that there are some disruption at the beginning and the end of PCR products sequencing . I think myself it's very difficult
Thank you very much