Hi, I want to make a buffer but I am not sure about the 25 mm HEPES/NH4OH (pH 8.0):
25 mm HEPES/NH4OH (pH 8.0), 20 mm KCl, 200 mm
NaCl, and 1% DMSO
I looked around for the a specific HEPES ammonia buffer but it is not available. I am assuming that the buffer is made of 25 mM HEPES and 25mM NH4OH but I am not sure and I want to do it right. Any ideas on how to make this 25 mm HEPES/NH4OH (pH 8.0)?