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Contamination....


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3 replies to this topic

#1 gilberthdzjr

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Posted 02 October 2013 - 02:13 PM

Greetings everyone. So my co worker and I ordered some bacteria from Carolina, and the lately our Staphylococcus epidermidis has bacillus subtilis contamination... We are rehydrating our bacteria into steril nutrient agar and using only sterile syringes. I havent been working at this college laboratory very long but I wanted to take the initiative and look this up myself maybe there is something I am not doing right. Thank you.



#2 Phil Geis

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Posted 03 October 2013 - 12:58 AM

What protocol demands the use of syringes?

#3 SunsetSatine

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Posted 13 October 2013 - 05:24 PM

Is your isolate in a sterium bottle? That would be a reason to use a syringe...are you having to work anaerobically?



#4 Phil Geis

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Posted 14 October 2013 - 08:10 AM

Sorry - of course you meant from the original vial rehydrated, not subsequent subculture.  .

 

I'd contact the supplier.






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