Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

PCR Primer trouble

primers

Best Answer phage434, 02 October 2013 - 09:28 AM

It's possibly contaminated, but otherwise likely simply diluted somewhat. It may be usable if not contaminated. You won't be able to remove the ice, since the primer solution will be frozen (I'm assuming the primer was already dissolved).

Go to the full post


  • Please log in to reply
2 replies to this topic

#1 ptnoob

ptnoob

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 02 October 2013 - 08:25 AM

A small distraction...

 

I accidentally dropped an ice fragment inside a primer eppendorf tube while using the micropipette! 

 

The technicians stored it in the freezer (-20ºC).....is the primer still usable? Contaminated? Will I be able to remove the fragment with a small set of sterilized tweezers?  ohmy.png



#2 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,475 posts
251
Excellent

Posted 02 October 2013 - 09:28 AM   Best Answer

It's possibly contaminated, but otherwise likely simply diluted somewhat. It may be usable if not contaminated. You won't be able to remove the ice, since the primer solution will be frozen (I'm assuming the primer was already dissolved).



#3 ptnoob

ptnoob

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 08 November 2013 - 09:20 AM

I thought so too!

Fortunately I was able to work with the same primer from another dilution.

 

Thanks for the help!







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.