As an immunologist, Molecular biology and cloning comes difficult to me. I am having problems generating Lentivirus. Essentially what I want to do is combine my Packaging, Envelope and GFP together in HEKs and then Infect my Stem cells so they fluoresce Green but for I can't for the life of me get past the stage where I collect a viral supernatant thats successful! I have tried with Mirus transfection reagent and with the CACL method. I am at my wits end here and I can't afford to buy in a fully made Lentivirus.
Any helpful advice or information for me please?