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Problem with epithelial cell-like growth in hybridoma culture

hybridoma monoclonal antibodies

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#1 move



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Posted 02 October 2013 - 05:58 AM

I have established a number of hybridoma cell lines and from time to time I have problems with epithelial cell-like growth in my hybridoma cultures. These seem to have a clear negative impact on the clonal growth of the hybridoma cells. Does anyone have experience with similar problems? Where do these cells come from, is there any additive that stimulates and what can you do to avoid this?
Grateful for any tips and advices smile.png .

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