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Extra nucleotides in sequencing...

...in the vector

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3 replies to this topic

#1 rmbio

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Posted 01 October 2013 - 03:20 AM

I am trying to sequencing an empty vector. I got following result. Can anyone explain the presence of 8 extra nucleotides in the obtained sequence? Could it be becuase the next 8 nucleotides (black square) are similar to this extra stretch? Or are these really present in my sequence?

 

Untitled.png
image ru


Edited by rmbio, 01 October 2013 - 03:21 AM.


#2 phage434

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Excellent

Posted 01 October 2013 - 04:08 AM

It is hard to tell without the electropherogram, but i would suggest that they are likely to be really there. I trust sequencing more than the literature or notes from other researchers.



#3 rmbio

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Posted 01 October 2013 - 09:26 AM

It is hard to tell without the electropherogram, but i would suggest that they are likely to be really there. I trust sequencing more than the literature or notes from other researchers.

 

Hi,

Thanks for reply. Since there was sequence (identical) available from two clones, I did not bother to post electrogram. They look quite fine as below.

 

chromtgrm.png
 


Edited by rmbio, 01 October 2013 - 09:27 AM.


#4 phage434

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Excellent

Posted 01 October 2013 - 11:11 AM

Make that "definitely there."






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