HOW TO detect target gene
Posted 24 April 2004 - 05:43 AM
I am in a difficult situation now,that is to say,I have transfected my target gene into the cells using EGFP as a reporter gene. My question is how to detect the expression of my target gene addition to the observation of EGFP.By the way,I have no antibody to target protein.
Thank you very much!
Posted 24 April 2004 - 09:16 PM
Posted 25 April 2004 - 05:16 AM
Another question is : Can I do W-B using antibody against the reporter gene EGFP though it can be detected by the eye?
Posted 25 April 2004 - 09:00 AM
Anyway it is up to you: what equipments are available and which technique you are more cofindent with?
Hope this help,
Posted 26 April 2004 - 03:51 AM
Thank you for your advice. And the equipments are no problem!