Does anyone have experience and success with detecting mouse BRCA1 by western blot? BRCA1 antibody datasheets indicate the protein should be around 220kd. I feel this specific to human because I think mouse is actually a smaller protein. I feel that mouse BRCA1 migrates around 200-210kd because I a using a new antibody with less background and the predominant band is around 205kd. Most antibodies produce a lot of non-specific bands so I am trying to figure out which band is correct. I have knocked down BRCA1 but with so many non-specific bands and not knowing where mouse BRCA1 migrates, it is hard to determine which band is correct. I also load at least 50ug protein fro whole cell lysate and have used nuclear lysates too.