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Genbank, 5' or 3'

molecular biology

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#1 FreddieP

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Posted 26 September 2013 - 03:36 PM

Hi, How do I figure out whether a sequence starts at the 5' or 3' end on Genbank? Thanks, I'm going crazy trying to find this out.



#2 phage434

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Posted 26 September 2013 - 04:17 PM

Unless it is explicitly shown otherwise, all sequence, everywhere, is shown 5' to 3'. Of course, if it is sequence of a double stranded piece of DNA, then there is also a reverse complement strand, which will (if written out) be in the 3' to 5' direction. Usually this strand is not shown, and is implicit.







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