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Splinkerette PCR


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#1 guido53

guido53

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Posted 25 September 2013 - 11:47 AM

Hi everybody, has anyone experience on splinkerette pcr? I'd like to use it to determine the copy number of a lentiviral vector.....I found in the net the protocol but I have some questions about it:

Are the enzymes always the same or  the second one is depending to the lentiviral LTR?

The only thing I have to know is the LTR sequence?

Thank you very much for the help =)






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