Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

storage of BL21 at -20


  • Please log in to reply
1 reply to this topic

#1 sune1972

sune1972

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 24 September 2013 - 10:08 PM

Hi I 

Due to logistical problems I wish to store my E. Coli BL21 at -20C instead of -80 for a period of 4 months.

Am I likely to experience a drop in viability, or would it be OK?

Best 



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,826 posts
413
Excellent

Posted 25 September 2013 - 01:19 AM

For glycerol stocks, yes, there will be a big drop off in viability.  -20 does not work for storage of glycerol stocks as they do not freeze.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.